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. 2021 Jun 15;148(12):dev199281. doi: 10.1242/dev.199281

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Laminin α5 is needed for HR⁺ luminal epithelial cell identity. (A) Representative CD24/CD29 FACS plot showing relative amounts of lineage marker negative luminal and basal MECs from 10-week-old Lama5^fl/fl;K8-CreERT2 and control animals. Gating strategy for the same sample is shown in Fig. S3A. (B,C) Quantification of the percentages of basal and luminal MECs (+/+, n=3; fl/fl, n=3 individuals analyzed) and (C) luminal HR⁺ and HR⁻ subpopulations from 10-week-old Lama5^fl/fl;K8-CreERT2 and control mice (+/+, n=6; fl/fl, n=6 individuals analyzed). Data points indicate samples analyzed from individual mice. (D) Mean intensity of Sca1⁺ luminal population from Lama5^fl/fl;K8-CreERT2 and control mice. Data show mean from n=5 separate experiments, in which 3-4 individual animals were analyzed per group. Data points indicate mean values from five separate experiments. (E) Representative Sca1-BV711 histograms of individual Lama5^+/+;K8-CreERT2 and Lama5^fl/fl;K8-CreERT2 mice. (F) qRT-PCR analysis of Esr1, PR and RANKL expression compared with GAPDH in luminal MECs from 10-week-old Lama5^fl/fl;K8-CreERT2 and control mice (Esr1 +/+, n=5; fl/fl, n=4; PR +/+, n=9; fl/fl, n=8; RANKL +/+, n=4; fl/fl, n=3). Data points indicate samples analyzed from individual mice. Data are mean±s.d. *P<0.05, **P<0.01 [unpaired two-tailed Student's t-test (B,C,F); paired t-test (D)].