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. 2021 Jul 3;20:127. doi: 10.1186/s12934-021-01618-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Violin plot of genes (small dots) involved in the methanogenesis KEGG pathway (map00680) in each bin (arranged on the X-axis) and the unbinned gene collection. Only bins, which contain at least are plotted. Filling colors indicate taxonomy at Class level. Each dot represents a KEGG 5 methanogenesis genes orthologue (KO) in the respective bin. Colors of the dots indicate the p-value of the log₂FC difference between N2_MTR and H2_MTR samples. Horizontal dashed red lines mark the log₂FC thresholds for significantly different KOs (respective p-value < 0.05)