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. 2021 Jul 3;22:194. doi: 10.1186/s12931-021-01785-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3. — 3-DZNeP suppressed M1 macrophage polarization while promoted M2 phenotype in MH-S cells. A The levels of IL-1β, IL-6, TNF-α were measured by ELISA in the supernatants of LPS stimulated MH-S cells and IL-10 levels were qualified by ELISA in IL-4 stimulated MH-S cells with different dose of 3-DZNeP (0, 1, 2, 5, 10 µM). B The mRNA expression of iNOS, Arg-1 and FIZZ-1 was measured by quantitative PCR. C Representative Western blot depicting MH-S cell lysates probed for EZH2, H3K27me3, iNOS, Arg-1, FIZZ-1 and GAPDH. D Expression levels of EZH2 and H3K27me3 were quantified by densitometry and normalized using GAPDH. E Expression levels of iNOS, Arg-1 and FIZZ-1 were quantified by densitometry and normalized using GAPDH. All data are expressed as mean ± SEM. (* p < 0.05 vs. MH-S cells with 0 µM 3-DZNeP, ^#p < 0.05vs. MH-S cells with PBS determined by one-way ANOVA for multiple group comparisons)