Fig. 3.
Effects of knockdown of TIA-1 and TIAR on stability of TPD52 mRNA. a RNA degradation assay. siRNAs for TIA-1 and TIAR, and control siRNA were transfected into SAS cells. After the addition of actinomycin D (10 μg/ml) to the culture, the cells were incubated under hypoxic conditions for another 0, 1, 2, and 4 h. Then, total RNA was collected, and RT-qPCR was performed to detect TPD52 mRNA. The value at time 0 is designated as “1,” and relative values are shown. Bottom right: calculated half-lives (t1/2) of TPD52 mRNA are shown. b RIP assay. SAS cells were exposed to normoxia or hypoxia for 24 h. Then, the cells were subjected to RIP assays to detect TPD52 mRNA, using anti-TIA-1 or anti-TIAR antibodies. The value of normoxia is designated as “1,” and relative values are shown. *, p < 0.05. Experiments were repeated 3 times