FIGURE 2.

Target engagement and efficacy of MRI‐1867 in experimental model of HpsPF in pale ear mice. (A) Body weight change in Sc‐Bleo (60 U/kg)‐induced PF. (B) Gene expression of fibrosis marker collagen 1a (Col1a). (C) Hydroxyproline content of the left lung. (D) Ashcroft scoring from the Masson trichrome staining. Lung tissue gene expression of Nos2 (E) and Cnr1 (F). Levels of endocannabinoid AEA (G) and 2AG (H) in lung tissue. Masson trichrome staining (I). CB₁R (J) and iNOS (K) immunostainings from lung tissue sections from control and bleomycin (60 U/kg) challenged pale ear mice. Data represent mean ± SEM from 6 control (Ctrl, pale ear mice infused with saline instead of bleomycin), 4 HpsPF with bleomycin+vehicle at day 8 (Veh), 15 HpsPF with bleomycin+ vehicle at day 42 (Veh), and 11 HpsPF with bleomycin+MRI‐1867 (MRI‐1867) at day 42. Data were analyzed by one‐way ANOVA followed by Dunnett's multiple comparisons test. * (p < 0.05) indicates significant difference from the control group. # (p < 0.05) indicates significant difference from the HpsPF mice treated with vehicle (Veh) at 42 day