FIGURE 4.

Bleomycin‐induced elevation of endocannabinoids, truncated oxidized phospholipids, and TGFβ‐1 in BALF attenuated by MRI‐1867 treatment. Levels of AEA (A) and 2AG (B) in BALF of HpsPF mice and control. Correlation with forced expiratory volume 0.1 s (FEV_0.1) as pulmonary function tests (PFTs) and endocannabinoids (C) in BALF. (D) Chemical structures of surfactant phosphatidyl cholines PAPC and POPC and their truncated oxidized phosphatidyl cholines (POVPC, PGPC, ALDOPC, and PAzePC). Levels of POVPC (E), PGPC (F), ALDOPC (G), PAzePC (H), and TGFβ‐1 (I) in BALF of pale ear mice. Data represent mean ± SEM. n = 6 pale ear mice for control (Ctrl, pale ear mice infused with saline instead of bleomycin), n = 8 HpsPF mice for vehicle (Veh), and n = 6 HpsPF mice for MRI‐1867 (MRI‐1867)‐treated groups for endocannabinoid measurements. n = 6 HpsPF mice for control (Ctrl), n = 6 HpsPF mice for vehicle (Veh), and n = 5 HpsPF mice for MRI‐1867 (MRI‐1867)‐treated groups for oxidized phospholipids and TGFβ‐1 measurements. Data were analyzed by one‐way ANOVA followed by Dunnett's multiple comparisons test. * (p < 0.05) indicates significant difference from the control group. # (p < 0.05) indicates significant difference from the HpsPF mice treated with vehicle (Veh) at 42 day