FIGURE 7.

MRI‐1867 treatment reduces fibroblast activation and IL‐11 secretion via iNOS inhibition in primary lung fibroblasts derived from HPSPF patients. (A) Schematic presentation of primary fibroblast isolation and in vitro experimental conditions. Gene expression levels of (B) NOS2 and (C) CNR1 in control and HPSPF human lung fibroblasts, and (D) levels of endocannabinoids in the culture medium at 24 h after incubation in the absence or presence of TGFβ‐1 (100 ng/ml). Gene expressions of (E) ACTA2, (F) COL1A1, and (G) IL11 in fibroblasts, and (H) IL‐11 level in culture media at 24 h after treatment with MRI‐1867, rimonabant, and 1400W in the presence of TGFβ‐1 (100 ng/ml). Data represent mean ± SEM. n = 3 per group. Data were analyzed by one‐way ANOVA followed by Dunnett's multiple comparisons test. * (p < 0.05) indicates significant difference from the control group. # (p < 0.05) indicates significant difference from the TGFβ‐1+ vehicle group