Fig. 4. Suppressive effect of Wnt-C59 on lipopolysaccharide (LPS)-induced activation of the Wnt/β-catenin pathway in RAW264.7 murine macrophage cells.

Cells were treated with 0, 30, or 50 μM of Wnt-C59, followed by LPS stimulation at 0.1 μg/ml for 4 h. (A) Cellular protein levels were measured by Western blotting. Original uncut Western blot images were shown in Supplementary Data 2. β-Actin was used an equal loading control. (B–F) Protein band intensities were quantified using ImageJ. *p < 0.05, **p < 0.01, ***p < 0.001 compared with cells stimulated with LPS with 0 μM of Wnt-C59. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared with unstimulated cells. Experiments were conducted in triplicate. Data are shown as mean ± standard deviation, and statistical significance was measured by unpaired t-test. p-LRP6, anti-phospho LRP6; p-GSK-3β, anti-phospho GSK-3β; p-β-catenin, anti-phospho β-catenin.