Fig. 1.

Effects of PLX4720 on cell growth and autophagy in cells with different BRAF mutational status. (A) Whole-cell lysates were immunoblotted with antibodies against phospho-ERK, ERK, phospho-MEK and MEK. Cells were BRAF homozygous or heterozygous for wild-type (WT), or V600E (E) mutation. (B) Cell viability, measured through WST-1 assay, relative to vehicle controls in four cell lines exposed to PLX4720 (0.1-10 μM) for 72 h. Values represent the mean ± SD of quadruplicate determinants from one of three representative experiments.