Table 1.
Strains and plasmids used in this study.
| Strains/plasmids |
Description |
Source or reference |
|---|---|---|
| Strains | ||
| Moorella thermoacetica | acetogenic bacterium | [33] |
| BL27 | MG1655 F-lambda-ilvG-rfb-50 rph-1 ΔhsdR ΔampC lacZ∷T7 | From Prof Quan |
| LNY01 | BL27 Ptrc-mut-ackA-pta | This study |
| LNY02 | BL27 ΔfadR | This study |
| LNY03 | BL27 PR-sdh | This study |
| LNY04 | BL27 Ptrc-mut-ackA-pta ΔfadR | This study |
| LNY05 | BL27 Ptrc-mut-ackA-pta PR-sdh | This study |
| LNY06 | BL27 ΔfadR PR-sdh | This study |
| LNY07 | BL27 Ptrc-mut-ackA-pta ΔfadR PR-sdh | This study |
| BL27 (MDA) | BL27 containing pET28a-MDA | This study |
| BL27 (M*DA) | BL27 containing pET28a-M*DA | This study |
| LNY01(M*DA) | LNY01 containing pET28a-M*DA | This study |
| LNY02(M*DA) | LNY02 containing pET28a-M*DA | This study |
| LNY03(M*DA) | LNY03 containing pET28a-M*DA | This study |
| LNY04(M*DA) | LNY04 containing pET28a-M*DA | This study |
| LNY05(M*DA) | LNY05 containing pET28a-M*DA | This study |
| LNY06(M*DA) | LNY06 containing pET28a-M*DA | This study |
| LNY07(M*DA) | LNY07 containing pET28a-M*DA | This study |
| Plasmids | ||
| pKD4 | oriR6Kγ, KmR, rgnB (Ter) | [32] |
| pKD46 | araBp-gam-bet-exo, bla (ApR), repA101 (ts), oriR101 | [32] |
| pCP20 | ApR, CmR, FLP recombinance | [32] |
| pBAD33 | Cloning vector, CmR, pACYC18 origin vector | Lab collection |
| pET28a-mcr-mut | KanR, pET-28a containing mutated mcr (N940V, K1106W and S1114R) gene from C.aurantiacus | This study |
| pET28a-M*DA | KanR, pET-28a containing codon-optimized mcr (N940V, K1106W and S1114R) gene from C.aurantiacus, dtsR1 and accBC genes from C.glutamicum | This study |
| pET28a-MDA | KanR, pET-28a containing codon-optimized mcr gene from C.aurantiacus, dtsR1 and accBC genes from C.glutamicum | [38] |