Table 1.
Antibodies used in this study
| Primary or secondary | Antibody | Supplier | Species | Dilution | Buffer solution | Incubation time | Temperature (°C) |
|---|---|---|---|---|---|---|---|
| Primary | Laminin (L9393) | Sigma-Aldrich (St. Louis, Missouri, U.S.A.) | Rabbit | 1:1000 | 3% BSA | overnight | 4 |
| Primary | Ki67 (Ab16667) | Abcam (Cambridge, England) | Rabbit | 1:500 | 3% BSA | overnight | 4 |
| Primary | Pax7 (Pax7) | DSHB (Iowa City, U.S.A.) | Mouse | 1:5 | 3% BSA | overnight | 4 |
| Primary | MyHC-1 (A4.480) | DSHB (Iowa City, U.S.A.) | Mouse | 1:20 | 3% BSA | overnight | 4 |
| Primary | MyHC-2A (SC-71) | DSHB (Iowa City, U.S.A.) | Mouse | 1:20 | 3% BSA | overnight | 4 |
| Primary | MyHC-2X (6H1) | DSHB (Iowa City, U.S.A.) | Mouse | 1:5 | 3% BSA | overnight | 4 |
| Primary | MyHC-2B (BF-F3) | DSHB (Iowa City, U.S.A.) | Mouse | 1:10 | 3% BSA | overnight | 4 |
| Secondary | TRITC (ZF-0313) | ZSGB-BIO (Beijing, China) | Anti-Mouse | 1:100 | 3% BSA | 1 h | 23 |
| Secondary | FITC (ZF-0311) | ZSGB-BIO (Beijing, China) | Anti-rabbit | 1:100 | 3% BSA | 1 h | 23 |
| Secondary | TRITC (ZF-0316) | ZSGB-BIO (Beijing, China) | Anti-rabbit | 1:100 | 3% BSA | 1 h | 23 |