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. Author manuscript; available in PMC: 2022 Aug 6.
Published in final edited form as: Biochem Biophys Res Commun. 2021 Jun 5;565:91–96. doi: 10.1016/j.bbrc.2021.05.082

Figure 4. TGF-β1 regulates the activation of Smad2/3.

Figure 4.

(A) Whole-mount immunostaining of phosphorylated Smad2/3 (pSmad2/3) at 6 hpa. Scale bar, 100 μm. (B) Quantitative fluorescence intensities of pSmad2/3 immunostaining in regenerating tails. The vertical axis indicates the average fluorescence intensity in regenerating tails of tgfb1 KO tadpoles normalized against tyrosinase KO control tadpoles. White arrowheads indicate amputation sites. White arrows indicate the localization of fluorescence signals in the regenerating tail tip. *P < 0.05.