FIGURE 1.

Lack of SdiA affects cell division and leads to morphological alterations of K. pneumoniae cells. (A) Growth curves of the wild-type, mutant (sdiA::kan^R) and complemented (sdiA::kan^R_comp) strains are displayed as log scale of the optical density of the cultures of each strain measured at the indicated times. No differences in growth curves were observed among the strains. (B,C) Aliquots of the cultures of each strain were submitted to optical microscopy analyses to investigate the morphology of the bacterial cells. Disruption of sdiA led the mutant strain to exhibit a filamentous phenotype. The mutant strain recover the same morphology of the wild-type and complemented strains only at high cell densities (O.D._{600 nm} of 1.0).