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. 2021 Jun 21;12:597735. doi: 10.3389/fmicb.2021.597735

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Copyright © 2021 Pacheco, Gomes, Siqueira, Assoni, Darrieux, Venter and Ferraz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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K. pneumoniae deprived of SdiA presents up-regulation of fimA (type 1 fimbriae) and down-regulation of mrkA (type 3 fimbriae). No change in the expression of ecpA (common pillus) was observed between the wild-type and mutant strains. The expression of the genes was analyzed by RT-qPCR on the wild-type and mutant K. pneumoniae strains cultured at O.D.₆₀_0n_m of 0.6. Error bars represent the standard deviation. Statistically significant differences between the strains were analyzed by multiple t-test (*p < 0.05; ***p < 0.001).