Fig. 1. Comparison of sperm concentration results between manual semen analysis (MSA), X1 PRO analyzer, and HT IVOS CASA. (A–C) Passing–Bablok regression plots comparing sperm concentration as obtained by MSA, X1 PRO, and HT CASA did not show significant deviation from linearity (p>0.05). The solid blue line represents the regression line, the red dashed line represents the diagonal line, and the brown dashed line represents a confidence band (n=101). (D–F) Bland–Altman analysis of the concentration deviation results between the MSA, X1 PRO, and HT CASA. The solid blue line represents the mean of the two methods and the red dashed lines are the 95% confidence ranges. SD: standard deviation.