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. 2021 Jun 21;9:685873. doi: 10.3389/fcell.2021.685873

FIGURE 2.

FIGURE 2

hAFSC-exo accelerated the wound healing rate and improved the regeneration quality in the full-thickness cutaneous-wounded rats. (A) Schematic representation of the experimental design. The full-thickness cutaneous-wounded model (12 mm diameter) was established on the shaved skin of the dorsal region. The rats were then administered treatments by local injection every 7 days: CTRL (PBS), hAFSC (2 × 106 cells), and hAFSC-exo (20 μg). (B) Morphological changes in the wound healing process (scale bar = 5 mm). (C) Changes in wound area during wound healing. (D) H&E and Masson staining of the healing tissues; scale bar (red) = 1 mm; scale bar (black) = 200 μm. (E–G) Number of hair follicles/field (20×), epidermal thickness, and percentage of collagen fibers (blue area in Masson staining) in the healing tissue according to histology. *P < 0.05; **P < 0.01; ***P < 0.001; mean ± SD; n = 5. CTRL, control; DPW, days post-wounding.