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. 2021 Jun 21;8:696262. doi: 10.3389/fvets.2021.696262

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Copyright © 2021 Li, Wang, Zhang, Tang, Wang, Liu, Qian, Zhu, Chen and Tan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Detection of Mycoplasma hyopneumoniae-specific P36 (948 bp) and 16S RNA (627 bp) genes for the different passage strains: M, DNA marker 2,000; lane 1, ES-2 (P1); lane 2, P40; lane 3, P80; lane 4, P120; lane 5, P160; lane 6, ES-2L (P200); lane 7, positive control; lane 8, negative control. (B) Morphological observation of strain ES-2L and ES-2 on solid medium using a low-power microscope; the colonies of strain ES-2 and ES-2L showed the characteristics of rounded, distinct edges and centers with granules, as indicated by the red arrow. (C) Morphological examination of strains ES-2L and ES-2 observed by transmission electron microscopy, indicating that strain ES-2 had no cell walls, cell membranes were surrounded by capsules, and the cytoplasmic structure was loose; characteristics of strain ES-2L were similar to those of strain ES-2, but there were a few large-celled mutant individuals, as indicated by the red arrow.