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. 2021 Jun 21;8:696262. doi: 10.3389/fvets.2021.696262

Figure 2.

Figure 2

(A) Change of average daily weight gain in each group; data are presented as the means ± SD (n = 4); **P < 0.01, Student's t-test. (B) Observation of lung tissue macroscopic lesions in each group; all pigs in the P1 group had typical “red consolidations” lesions of lung tissue (indicated by the red arrow). None of the pigs in the ES-2L group showed typical lesions. (C) Detection of P36 gene (948 bp) by PCR assay for pig's lungs of each group: M, DNA marker 2,000; lanes 1, 2, 3, and 4 correspond to P36 gene on ES-2 group; lanes 5, 6, 7, and 8 correspond to P36 gene on ES-2L group; lanes 9, 10, 11, and 12 correspond to P36 gene on P120 group; lanes 13, 14, 15, and 16 correspond to P36 gene on P80 group; lanes 17, 18, 19, and 20 correspond to P36 gene on control group; lane 21, positive control; lane 22, negative control. (D) Examination of lung tissue hematoxylin and eosin staining by microscopy at 100-fold magnification; the red arrow indicates that there were severe mononuclear cell and lymphocyte infiltration and alveolar consolidation in lung tissue.