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. 2021 Jun 21;9:645368. doi: 10.3389/fcell.2021.645368

FIGURE 1.

FIGURE 1

Creation and validation of Ulk4 knockout (KO) mice. (A) Diagram of the construct used to generate Ulk4 hypomorph mice. Note that the first seven exons were supposed to remain after successful homologous recombination. (B,C) Ulk4 mRNA expression was determined by RT-qPCR using two pairs of primers that recognize exons 1 and 15 of Ulk4. Compared to wild-type (WT) littermates, Ulk4 hypomorph mice exhibited comparable amounts of exon 1 (B) (control, 87 ± 13%; hypomorph, 101 ± 2%, p > 0.05, n = 4 each), but significantly low levels of exon 15 (C) (control, 91 ± 12%; KO, 6.6 ± 0.8%, **p < 0.01, n = 4 each, Student’ t-test), indicating a successful homologous recombination. (D) Ulk4 knockout mice presented a significantly reduced body size at postnatal day 7 (P7). (E) Ulk4 hypomorph mice displayed significantly lighter body weight than their littermates after P4, suggesting a marked growth retardation (N = 6 in each genotype). ∗∗p < 0.01, two-way repeated ANOVA.