FIGURE 7.

Targeted deletion of Ulk4 in neural stem cells recapitulates the phenotype of thinner cortex in conditional knockout (CKO) mice. (A–J) Similar to the phenotypes observed in hypomorph mice, staining using layer-specific markers showed that the thickness of layers II–III, and V was also reduced in the cortex of Ulk4^nestin CKO mice, as shown by Hoechst (A,F) and Cux2 (B,G) staining and plxnD1 expression (D,I), whereas the thickness of Rorβ⁺ layer IV (C,H) and TLE4⁺ layer VI (E,J) was unaltered. N = 4 each, ^∗p < 0.05, Student’s t-test. Scale bar, 50 μm.