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. 2021 Jun 30;13:5177–5185. doi: 10.2147/CMAR.S304869

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© 2021 He et al.

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The methylated state of AGR2 in LUAD cells. (A) MSP results of AGR2 in both LUAD cell lines and normal cell line. U refers to the unmethylated alleles, M refers to the methylated alleles); (B) qRT-PCR was used to detect the mRNA expression level of AGR2 after 95-D and H1299 cells were treated with different doses of DNA methyltransferase inhibitor 5-AzadC; (C) Western blotting assessed protein expression of AGR2 in 95-D and H1299 cells after treatment of different doses of DNA methyltransferase inhibitor 5-AzadC. *P<0.05.