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. 2021 Jun 21;12:685546. doi: 10.3389/fimmu.2021.685546

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Copyright © 2021 Ba, Peng, Cheng, Lin, Li, He, Li, Wang and Qin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirmation of talaromyces marneffei specific amplification from plasma by next-generation sequencing. (A) shows the reads mapped to talaromyces marneffei derived from NGS data. A total of 248 reads mapped to talaromyces marneffei in the reference database which contains about 8000 pathogen genomes, and got a total coverage of 0.0085% respectively. (B) shows the distribution of bacterial sequences (N = 529 reads) identified in the patient’s plasma included Talaromyces marneffei (N = 248;47%), Cutibacterium, Acinetobacter, Staphylococcus, Acidovorax, Corynebacterium, Malassezia.