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. 2021 Jun 21;12:649140. doi: 10.3389/fgene.2021.649140

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Copyright © 2021 Wang, Li, Cao, Xiao, Liu, Jin and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ACAA1 knockdown increases lipid accumulation and the triglyceride content during sheep preadipocyte differentiation. (A) Determination of the transfection efficiency of siRNA-ACAA1 (200×). (B) After 8 days of differentiation, the cellular lipid droplets were stained with oil red O (200×). (C) ACAA1 mRNA expression was detected after siRNA-ACAA1 or NC transfection on days 0, 4, and 8 of adipogenic differentiation. The siRNA-ACAA1 groups all showed significantly lower expressions than the control group (**p < 0.01). (D) The triglyceride content after 8 days of induction in the siRNA-ACAA1 group was significantly higher than that in the control group (**p < 0.01). (E) The lipid droplet content measured at 490 nm after 8 days of induction was significantly higher in the siRNA-ACAA1 group than that in the control group (**p < 0.01). siRNA, small interfering RNA; NC, negative control.