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. 2021 Mar 3;12(2):395–425. doi: 10.1016/j.jcmgh.2021.02.013

Figure 2.

Figure 2

GECs express Rev-erbα during H pylori infection. (A) Representative immunofluorescence staining images showing Rev-erbα expression (red) in cytoplasm and nucleus (arrow) of CD326+ cells (green) and Rev-erbα–expressing (red) pepsinogen II+ chief cells (green) in gastric corpus of H pylori–infected patients. Scale bars: 10 μm or 100 μm. (B) Representative immunofluorescence staining images showing Rev-erbα–expressing (red) cells and H pylori (green) colonization in gastric mucosa of H pylori–infected patients. Scale bars: 10 μm. (C) Representative immunofluorescence staining images showing Rev-erbα expression (red) in WT H pylori–infected, and uninfected AGS cells (MOI = 100, 6 hours). Scale bars: 1 μm. (D) Rev-erbα expression in the nucleus of WT H pylori–infected, and uninfected AGS cells (MOI = 100, 24 hours) was analyzed by Western blot. The expression of cagA protein in AGS cells infected with WT H pylori or ΔcagA (MOI = 100, 24 hours) was analyzed by Western blot. (E, F) Rev-erbα mRNA expression and Rev-erbα protein in WT H pylori–infected and (E) uninfected AGS cells or (F) human primary GECs at different time point (MOI = 100) or with different MOI (24 hours) were analyzed by real-time PCR or Western blot (n = 3). (G) Rev-erbα mRNA expression and Rev-erbα protein in WT H pylori–infected, ΔcagA-infected, and uninfected AGS cells or human primary GECs (MOI = 100, 24 hours) were analyzed by real-time PCR and Western blot (n = 3). (H) Rev-erbα expression in WT H pylori–infected, ΔcagA-infected, and uninfected GES-1 cells, HGC-27 cells, SGC-7901 cells, and BGC-823 cells (MOI = 100, 24 hours) was analyzed by real-time PCR and Western blot (n = 3). ∗P < .05, ∗∗P < .01 for groups connected by horizontal lines.