Skip to main content
. 2021 Mar 3;12(2):395–425. doi: 10.1016/j.jcmgh.2021.02.013

Figure 8.

Figure 8

Rev-erbα inhibits CD45+CD11c–Ly6G–CD11b+CD68–myeloid cell accumulation in gastric mucosa during H pylori infection. (A) Representative dot plots of CD45+CD11c+Ly6G– dendritic cells, CD45+CD11c–Ly6G+ neutrophils, CD45+CD11c–Ly6G–CD3+NK1.1– T cells, CD45+CD11c–Ly6G–CD3–NK1.1+ natural killer cells, CD45+CD11c–Ly6G–CD3–NK1.1–CD19+ B cells, and CD45+CD11c–Ly6G–CD11b+CD68– myeloid cells by gating on CD45+ cells in gastric mucosa of WT H pylori–infected mice 8 weeks p.i.. Representative Giemsa staining images showing FACS-sorted CD45+CD11c–Ly6G–CD11b+CD68– myeloid cells in gastric mucosa of WT H pylori–infected WT mice 8 weeks p.i.. (B) The levels or numbers of CD45+CD11c+Ly6G– dendritic cells, CD45+CD11c–Ly6G+ neutrophils, CD45+CD11c–Ly6G–CD3+NK1.1– T cells, CD45+CD11c–Ly6G–CD3–NK1.1+ natural killer cells, CD45+CD11c–Ly6G–CD3–NK1.1–CD19+ B cells, and CD45+CD11c–Ly6G–CD11b+CD68– myeloid cells in gastric mucosa of WT H pylori–infected WT and Rev-erbα–/– mice were compared 8 weeks p.i. (n = 5). (C–E) CD45+CD11c–Ly6G–CD11b+CD68– myeloid cell levels or CD45+CD11c–Ly6G–CD11b+CD68– myeloid cell numbers in (C) gastric mucosa of WT H pylori–infected WT and Rev-erbα–/– mice, (D) gastric mucosa of WT H pylori–infected mice injected with Rev-erbα agonist SR9009 or cremophor control, or Rev-erbα antagonist SR8278 or DMSO control, or (E) gastric mucosa of WT H pylori–infected BM chimera mice were compared 8 weeks p.i. (n = 5). (F) Representative immunofluorescence staining images showed Ly6C+CD11b+ myeloid cell infiltration in gastric mucosa of WT H pylori–infected WT and Rev-erbα–/– mice 8 week p.i.. Scale bars: 20 μm. ∗∗P < .01, n.s. P > .05 for groups connected by horizontal lines.