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Primers for ChIP PCR analysis
| Gene | Primer | Sequence 5′→3′ |
|---|---|---|
| β-defensin-1 | forward | AGCAAGGAAAGCTGTGTTTCGG |
| reverse | GATGCAGTGGGATCCTGTAGCT | |
| CCL21 | forward | GGAGCGTAGTGAGGAGACAGT |
| reverse | GGGCCTGAGCCTTCCTTCTAT |
NOTE. Primers for ChIP analysis of β-defensin-1 and CCL21 were designed and produced by Sangon Biotech (Shanghai, China). Primers for Rev-erbα purchased in Cell Signaling Technology pathway (Cat#:13413).
ChIP, chromatin immunoprecipitation; PCR, polymerase chain reaction.
