Skip to main content
. 2021 Mar 2;12(2):465–487. doi: 10.1016/j.jcmgh.2021.02.010

Figure 2.

Figure 2

S1-tag knock-in did not affect NEAT1_2 expression, proliferation, or apoptosis of HCC cells. (A) Diagram of RIP-seq. (B) RIP analysis of NEAT1_2 relative expression in S1-tagged HCCML3 or SKhep1 cells with streptavidin (SA). Endogenous expression (Endo-exp) of NEAT1_2 was detected by qRT-PCR. (C) Proliferation of WT or S1-tagged HCCLM3 or SKhep1 cells was detected by MTT assay. (D) Apoptosis of WT or S1-tagged HCCLM3 or SKhep1 cells was detected by flow cytometry. Data are represented as means ± SD (n = 3; ∗P < .05, ∗∗P < .01). IP, intraperitoneal.