Fig. 6.

p‐Akt Thr308 regulated p‐eNOS Ser1177 expression in HUVECs exposed to different temperature. HUVECs were exposed to different temperatures (37, 22, and 4 °C) for 1 h; then the total and phosphorylated levels of Akt and eNOS were detected by western blot. (A) Western blot of the protein expressions of p‐Akt Ser473, p‐Akt Thr308, total‐Akt, p‐eNOS Ser1177, and total‐eNOS in HUVECs. (B) Quantitative analysis of the protein expressions of p‐Akt Ser473, p‐Akt Thr308, total‐Akt, p‐eNOS Ser1177, and total‐eNOS in HUVECs. The relative levels of p‐Akt Ser473, p‐Akt Thr308, p‐eNOS Ser1177 were quantified as the ratios of p‐Akt Ser473/Akt, p‐Akt Thr308/Akt, and p‐eNOS Ser1177/eNOS, respectively. Data were expressed as mean ± SE, n = 3. Data were analyzed with one‐way ANOVA followed by Tukey's multiple comparisons test. *P < 0.05 vs. 37 °C; **P < 0.01 vs. 37 °C; ***P < 0.001 vs. 37 °C.