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. 2021 May 19;3(7):531–540. doi: 10.1096/fba.2020-00066

FIGURE 4.

FIGURE 4

spE15 transcript is not present in mouse brain. A, Immunohistochemistry for spE15 in WT mouse choroid plexus. spE15; red, and KL1 in green. We Micro‐dissected choroid plexus from WT mouse brain were subjected to RT‐PCR using the different primers B. C, the primers (top panel), cDNA origin (middle panel) and RT‐PCR products in 1.2% agarose ethidium bromide gel (bottom panel). Lanes 2, 4, 6, 8, 10 showed strong transcript of Kl1, spKl1, and short spKl1, and very short transcripts of spKl1 and αKlotho respectively. Lanes 1, 3, 5, 7, 9, showed the transcripts in plasmids as PCR templates as positive control with. (* shows verified correct sequence.)