Fig. 2.

IgA responses to Env protein immunogen. The Env IgA sp act (ng anti-Env IgA antibody per µg total IgA) was calculated after measuring concentrations of Env-specific and total IgA by ELISA. The geometric mean sp act ×/÷ SEM is shown for nasal secretions (NS), cervicovaginal secretions (CVS), rectal secretions (RS), and sera. Note that NS were not collected for antibody analysis on days when IN immunization was performed with DNA/protein ± Invaplex_NAT (black arrows) or Ad-SIV (red arrow). Asterisks denote the wks when Gp 3 had significantly greater sp act than Gp 1. Crosses indicate when Gp 2 had significantly greater sp act than Gp 1 (all p ≤ 0.05 by Mann-Whitney). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)