Figure 2.

HMGB1 does not induce TGF-β secretion and galectin-9 expression in primary human AML cells lacking expressions of TLRs 2 and 4. Primary human AML cells lacking expression of TLRs 2 and 4 were exposed for 16 h to 2.5 µg/ml HMGB1 and TGF-β secretion and Smad3 activating phosphorylation (A), galectin-9 expression and secretion (B), expression of TLRs 2 and 4 as well as RAGE proteins (C) and Tim-3 protein expression and secretion (D) were analysed. Images are from one experiment representative of 4 which gave similar results. Quantitative data are shown as mean values ± SEM from four independent experiments.