Fig. 1.

(A) The spatiotemporal, transcriptomic, and morphological diversity of nIPCs remains almost entirely unexplored. (B) The GZs expand in thickness during neurogenesis. The EOMES⁺ area expands until GW19, after which it virtually disappears in the oSVZ and diminishes in the iSVZ, as shown in E. Distance measurements were taken from the ventricle to the outermost EOMES⁺ cell. Error bars represent technical variations from nine independent measurements of one biological sample. The GW20 measurements represent an average of nine values from both the occipital and prefrontal cortex. Error bars are SD. (C) From GW14 to GW18, most nIPCs are found within the iSVZ, not the oSVZ. However, in the oSVZ, nIPCs costain with KI67 at a higher rate, indicating that these nIPCs are more proliferative (C). The percentage of total EOMES in the oSVZ or iSVZ is the number of EOMES⁺ nuclei in that region over the total EOMES⁺ nuclei for a given length along the ventricle. The KI67 fraction represents the percentage of EOMES⁺ nuclei that also stain for KI67 in a given region for a given length along the ventricle. Error bars are SD. (D) The number of nIPCs expand from GW14 to GW18, during early neurogenesis. (E) Around GW19, the number of nIPCs abruptly declines. A thin band of EOMES⁺ cells remains in the iSVZ, and few or no nIPCs are found in the oSVZ. This later distribution was evident in three out of four samples from GW19 to GW20 and all three samples after GW20.