Fig. 8.

LC-MS analysis of in vivo activity of C5-MT, TET, GT-II, and GT-I of contigs 14 and 43. Nominal masses are labeled for each peak and further described in the text. Peak 1 (243 u) corresponds to 5mC. Peak 2 (257 u) corresponds to 5hmC. Peak 3 (460 u) was attributed to 5-GlcNAcßmC, peak 4 to 5-GlcßmC, peaks 5 and 5′ (622 u) to 5-GlcNAcGlcmC, peak 6 (581 u) to 5-GlcGlcmC, and peak 7 (663 u) to 5-GlcNAcGlcNAcmC. Anomeric configurations of disaccharides 581, 622, and 663 u were not determined. EV, empty vector and r, ribo form of the nucleoside.