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. 2021 Jun 21;12:662235. doi: 10.3389/fneur.2021.662235

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Copyright © 2021 Gabriel-Salazar, Lei, Grayston, Costa, Medina-Gutiérrez, Comabella, Montaner and Rosell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SVZ-derived NSCs responses to angiogenin: (A) timeline of the experimental procedure of neurosphere cultures and representative images; (B,C) box plots showing quantification of the NSC-forming neurospheres after angiogenin stimulation (100 or 200 ng/ml) at different time points, the inhibition with neomycin, and the largest neurosphere diameters obtained with angiogenin treatment; n = 4–9; **p < 0.001 and ***p < 0.001 vs. control. Box plots represent median (IQR) of the percentage vs. control values of each independent experiment. ANG/A, angiogenin; NSCs, neural stem cells; NMC, neomycin; NF, neurospheres.