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C16 preferentially modulated macrophages and independent on inducing macrophages apoptosis cells were incubated with 5 uM C16/SIN for 24 h, and then 500 ng/mL LPS challenged for another 6 h. Neutropils were separated from mice. (a) The mRNA levels of IL-1β and IL-6 were assayed by RT-qPCR. (b) Cells were stained with Annexin V/PI and measured by flow cytometry.
*P < 0.05. **P < 0.01. ***P < 0.001 versus control group.
