Table 5.

Comparison of DPAdSV and HPLC-DAD methods for determination of IDO activity (Kyn generated by IDO present in cellular extracts) or Kyn secreted into culturing medium collected from a culture of SK-OV-3 cells.

Sample type: cellular lysate
IDO activity ± SD [nmol Kyn/min/mg protein]
Sample	DPAdSV	HPLC–DAD	P value
1	1.19 ± 0.16	1.35 ± 0.15	0.349
2	4.48 ± 0.39	4.93 ± 0.18	0.233
3	0.63 ± 0.03	0.69 ± 0.03	0.086
4	0.73 ± 0.05	0.78 ± 0.07	0.411
Sample type: culturing medium from cancer cells
Kyn concentration ± SD [µM]
5	56.00 ± 8.29	60.65 ± 0.35	0.507
6	126.22 ± 3.58	130.783 ± 1.17	0.194
7	84.11 ± 1.76	84.04 ± 1.16	0.859
8	51.96 ± 7.05	55.38 ± 0.60	0.563
9	53.73 ± 2.11	53.54 ± 0.51	0.913
10	124.95 ± 4.72	122.38 ± 2.66	0.546

Abbreviation: SD, standard deviation from n = 3 (DPAdSV) or n = 2 (HPLC–DAD) measurements.