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. 2021 May 11;22(7):e52295. doi: 10.15252/embr.202052295

Figure EV2. Validation of hSgo1 KD efficiency and the conservation of the hSgo1 coiled‐coil region.

Figure EV2

  1. Validation of the hSgo1 antibody and the hSgo1 RNAi by immunoblotting. While the hSgo1 antibody detects unspecific bands in the whole cell lysates (see input), it is specific for hSgo1 after B56 IP, as the treatment with hSgo1 RNAi completely abolishes hSgo1 signal after 48h.
  2. Validation of the hSgo1 antibody and the hSgo1 RNAi by immunofluorescence. Representative immunofluorescent images are shown. Scale bar, 5 µm.
  3. The conservation of the hSgo1 coiled‐coil region. The residues mutated in Sgo1 3A and 4A are indicated.