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. 2021 May 28;22(7):e52154. doi: 10.15252/embr.202052154

Figure 4. Reduced GABAAR levels but not GlyRs at mixed inhibitory synapses by 4‐AP treatment.

Figure 4

  1. Triple immuno‐labeling of gephyrin, GlyRs, and GABAARs at mixed inhibitory synapses (arrow) imaged with conventional fluorescence microscopy. Scale bar: 5 µm.
  2. Quantification of the apparent synaptic area (in pixels) of gephyrin clusters (KS test P < 0.0001).
  3. Lower fluorescence intensity of GABAARs but only a minor reduction of GlyRs at synaptic gephyrin clusters (KS test, P < 0.0001, both panels).
  4. Reduced GABAAR/GlyR intensity ratio at mixed inhibitory synapses (KS test, P < 0.0001). Number of synapses: n = 9,416 in TTX and n = 6,949 in 4‐AP conditions from three independent experiments.

Source data are available online for this figure.