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. 2021 Jun 12;25(13):6032–6045. doi: 10.1111/jcmm.16460

FIGURE 5.

FIGURE 5

SPRED‐1 is implicated in CXCL12/miR‐126‐induced vascular tubes formation. (A) To determine the effect of miR‐126‐3p on SPRED‐1 expression, HUVEC were transfected with pre‐miR‐126‐3p for 24 h. Total proteins were extracted, and Western blot analysis was performed. (B) The inhibition of SPRED‐1 mRNA was checked by qRT‐PCR and using 18S rRNA as endogenous control (C) To analyse the implication of SPRED‐1 on CXCL12 induced vascular tubes formation in vitro, HUVEC were transected with siRNA‐SPRED‐1 at 25 nmol.L‐1 during 24 h. 7000 HUVECs were seeded on Matrigel and stimulated by CXCL12 at 6 nmol.L‐1 for 6 h. Vascular tubes formation was observed and the quantity of meshes was determined using phase contrast microscope and Archimed(TM) and Histolab(TM) software. Data were presented as the mean ± SEM of three and four independent experiments. **p <.01 vs Untreated cells; *p <.05 vs Untreated aortas