FIGURE 2.

Silencing of CIRBP inhibits ferroptosis in HK2 cells. HK2 cells were transfected with CIRBP siRNA or control vector and then treated with hypoxia for 6 h and reoxygenation for 12 h (H6R12) or erastin (10 μmol/L, 24 h). A, The expression of CIRBP was detected by western blot in HK2 cells. B and C, Cell viability was measured by Cell counting kit‐8 (CCK‐8) assay. D‐G, Ferroptosis‐associated markers, GSH, MDA, iron, and ROS levels were assayed. H, Mitochondrial morphological changes were observed by transmission electron microscopy. Values are expressed as means ± SD. n = 3, ^* P < .05, ^** P < .01