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. 2021 Jun 10;25(13):6203–6216. doi: 10.1111/jcmm.16567

FIGURE 5.

FIGURE 5

CIRBP induced ferritinophagy activation is inhibited by si‐ELAVL1. HK2 cells transfected with CIRBP or negative control (nc) vector were co‐transfected with si‐ELAVL1 or treated with hydroxychloroquine (HCQ, 50 μmol/L, 24 h), and then cultured under 6 h hypoxia and 12 h reoxygenation (H6R12). A, The expressions of CIRBP and ELAVL1 were confirmed after transfection. B, Representative blots of protein levels of FTH1, NCOA4, and LC3II/I. C, Quantification data of FTH1, NCOA4, and LC3II/I for each group. D, Representative immunoblotting and quantification data for LC3 II in the indicated cells together with BafA1 treatment. E, Representative immunofluorescence images of BECN1. Scale bar, 50 μm. F, Representative double‐immunofluorescence images of LC3B (red) and NCOA4 (green) by confocal microscopy. Magnification, 600×. Nuclei were stained with DAPI (blue). G, Representative double‐immunofluorescence images of ferritin (red) and LAMP2 (green) by confocal microscopy. Magnification, 600×. Nuclei were stained with DAPI (blue). The values are expressed as means ± SD. n = 3, *P < .05, **P < .01