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. 2021 Apr 7;25(13):5949–5962. doi: 10.1111/jcmm.16342

FIGURE 6.

FIGURE 6

KDM5B inhibitor GSK‐467 blocks HCC progression in vitro and in vivo. A, Cell sphere formation evaluated by cell sphere formation assay. B, Colony formation ability evaluated by clonogenic assay. C, Cell migration detected by scratch test. D, Cell invasion detected by Transwell assay. E, Weight of transplanted tumours. F, Growth curve of subcutaneous transplanted tumours. G, RT‐qPCR to detect the expression of KDM5B, miR‐448, YTHDF3 and ITGA6 in tumour tissues. H, Western blot analysis to detect the expression of KDM5B, YTHDF3 and ITGA6 in tumor tissues. I, Representative images showing xenografts in nude mice. J, Immunohistochemistry of KDM5B, YTHDF3 and ITGA6 proteins in tumour tissues (500×). The data in multiple groups were compared using one‐way ANOVA and Tukey's post hoc test. The data in groups at different time were analysed by repeated measures ANOVA followed by Bonferroni's post hoc test. *P < .05 when compared with the NS group. & P < .05 when compared with the GSK‐467 + oe‐NC group