Fig. 2. (a) Proposed enzymatic pathway for the conversion of PET to value added product vanillin. LCC: Leaf-branch compost cutinase; TPADO: terephthalate 1,2-dioxygenase; DCDDH: 1,4-dicarboxylic acid dehydrogenase; O-MT: O-methyltransferase; CAR: carboxylic acid reductase; FAD: flavin adenine dinucleotide; NAD(P)H: reduced nicotinamide adenine dinucleotide (phosphate); SAM: S-adenosyl-l-methionine (b) design of pathway enzyme expression constructs pVan1 and pVan2. (c) HPLC traces showing proof of concept for the pathway. Vanillin was only detected when TA was added to cells expressing pVan1, pVan2 and pSfp.