FIGURE 4.

MiR‐383‐5p reversed the effects of HULC on HCC progression and chemosensitivity of Oxa. (A) Transfection efficiency of miR‐383‐5p mimic in Hep3B cells and transfection efficiency of miR‐383‐5p inhibitor in Huh7 cells were measured by qRT‐PCR (t‐test). (B–F) Hep3B cells were transfected with Vector, oe‐HULC, oe‐HULC + mimic NC, or oe‐HULC + miR‐383‐5p mimic and Huh7 cells were transfected with si‐NC, si‐HULC, si‐HULC + inhibitor NC, si‐HULC + miR‐383‐5p inhibitor, and then these cells were treated with different concentrations of Oxa (B,C) or 6 μM Oxa (D–F). (B,C) CCK‐8 assay was conducted to determine the cell viability and IC50 of Oxa value (ANOVA). (D) The number of colonies was examined by colony formation assay (ANOVA). (E) Cell apoptosis was determined using flow cytometry analysis (ANOVA). (F) Western blot assay was performed to measure the protein levels of cyclinD1 cleaved‐caspase‐3, LC3I/II, and p62 (ANOVA). *p < .05. ANOVA, analysis of variance; CCK‐8, Cell Counting Kit‐8; HCC, hepatocellular carcinoma; HULC, highly upregulated in liver cancer; LC3, light Chain 3; NC, negative control; qRT‐PCR, quantitative real‐time polymerase chain reaction