FIGURE 7.

HULC positively regulated VAMP2 expression by sponging miR‐383‐5p in HCC cells. (A) The mRNA expression and protein expression of VAMP2 were determined by qRT‐PCR and western blot analyses, respectively, in Hep3B cells transfected with Vector, oe‐HULC, oe‐HULC + mimic NC, or oe‐HULC + miR‐383‐5p mimic (ANOVA). (B) QRT‐PCR and western blot analyses were conducted to analyze the mRNA level and protein level of VAMP2 in Huh7 cells transfected with si‐NC, si‐HULC, si‐HULC + inhibitor NC, or si‐HULC + miR‐383‐5p inhibitor (ANOVA). *p < .05. ANOVA, analysis of variance; HCC, hepatocellular carcinoma; HULC, highly upregulated in liver cancer; NC, negative control; qRT‐PCR, quantitative real‐time polymerase chain reaction; VAMP2, vesicle‐associated membrane protein‐2