FIGURE 8.

Upregulation of HULC promoted tumor growth and inhibited chemosensitivity of Oxa by regulating miR‐383‐5p and VAMP2. Hep3B cells transfected with Vector or oe‐HULC were inoculated subcutaneously into the nude mice. The mice were treated with Oxa (5 mg/kg) twice a week after injection for 3 days. (A,B) Tumor volume and weight were measured (ANOVA). (C) The expression levels of HULC, miR‐383‐5p, and VAMP2 were examined by qRT‐PCR in resected tumor tissues (t‐test). (D) Western blot assay was applied to detect the protein abundance of VAMP2 in resected tumor masses (t‐test). (E) The protein levels of cyclinD1, cleaved‐caspase‐3, LC3I/II, and p62 were analyzed in resected tumor tissues using western blot analysis (t‐test). *p < .05. ANOVA, analysis of variance; HULC, highly upregulated in liver cancer; LC3, light Chain 3; qRT‐PCR, quantitative real‐time polymerase chain reaction; VAMP2, vesicle‐associated membrane protein‐2