Table 1.
Overview of glutathionylation sites on plastid proteins and evolutionary conservation
| Protein name | Cys | Org. | Cat. | Ref. | |
|---|---|---|---|---|---|
| Redox regulation | 2-Cys peroxiredoxin | 172 | Ps | yes | Calderón et al. 2017 [92] |
| Glutaredoxin S12 (GRXS12) | 29 | Pt | yes | Zaffagnini et al. 2012 [73] | |
| Thioredoxin f (TRX-f) | 60 | At | no | Michelet et al. 2005 [31] | |
| Photosynthesis | Ferredoxin 1 | 48 | Cr | no | Zaffagnini et al. 2012 [73] |
| 69 | Cr | yes | Zaffagnini et al. 2012 [73] | ||
| Fructose-1,6-bisphosphatase | 109 | Cr | no | Zaffagnini et al. 2012 [73] | |
| Fructose-1,6-bisphosphate aldolase | 58 | Cr | no | Zaffagnini et al. 2012 [73] | |
| Glyceraldehyde-3-phosphate dehydrogenase, A subunit (GAPA) | 156 | At | yes | Zaffagnini et al. 2007 [93] | |
| Plastocyanin | 130 | Cr | yes | Zaffagnini et al. 2012 [73] | |
| Phosphoglycerate kinase | 159 | Cr | no | Zaffagnini et al. 2012 [73] | |
| 412 | Cr | no | Zaffagnini et al. 2012 [73] | ||
| Phosphoribulokinase | 47 | Cr | no | Zaffagnini et al. 2012 [73] | |
| 274 | Cr | no | Zaffagnini et al. 2012 [73] | ||
| Photosystem II (PSII) core phosphatase (PBCP) | 168 | Os | no | Liu et al. 2019 [79] | |
| 176 | Os | no | Liu et al. 2019 [79] | ||
| 195 | Os | no | Liu et al. 2019 [79] | ||
| Ribulose bisphosphate carboxylase large chain | 172 | Cr | no | Zaffagnini et al. 2012 [73] | |
| 247 | Cr | no | Zaffagnini et al. 2012 [73] | ||
| 427 | Cr | no | Zaffagnini et al. 2012 [73] | ||
| Transketolase | 84 | Cr | no | Zaffagnini et al. 2012 [73] | |
| Triose phosphate isomerase (chloro TPI) | 15 | At | no | López-Castillo et al. 2016 [80] | |
| Carbohydrate metabolism | ADP-glucose pyrophosphorylase large subunit | 112 | Cr | no | Zaffagnini et al. 2012 [73] |
| Alpha-amylase 3 (AMY3) | 499 | At | yes | Gurrieri et al. 2019 [77] | |
| 587 | At | yes | Gurrieri et al. 2019 [77] | ||
| Beta-amylase 3 (BAM3) | 433 | At | no | Storm et al. 2018 [78] | |
| Biosynthesis | Acetohydroxy acid isomeroreductase | 439 | Cr | no | Zaffagnini et al. 2012 [73] |
| Full-length thiazole biosynthetic enzyme | 106 | Cr | no | Zaffagnini et al. 2012 [73] | |
| Isopropylmalate dehydratase, large subunit | 444 | Cr | no | Zaffagnini et al. 2012 [73] | |
| Magnesium-chelatase subunit chlI | 184 | Cr | no | Zaffagnini et al. 2012 [73] | |
| others | 3′-phosphoadenosine 5′-phosphate phosphatase SAL1 | 119 | At | no | Chan et al. 2016 [76] |
| 190 | At | no | Chan et al. 2016 [76] | ||
| Chaperonin 60B2 | 249 | Cr | no | Zaffagnini et al. 2012 [73] | |
| 537 | Cr | no | Zaffagnini et al. 2012 [73] | ||
| Heat shock protein 70B (HSP70B) | 349 | Cr | no | Michelet et al. 2008 [75] | |
| Phosphorylase | 171 | Cr | no | Zaffagnini et al. 2012 [73] | |
| Protein tyrosine phosphatases (PTP) | 78 | At | no | Dixon et al. 2005 [72] | |
| 176 | At | no | Dixon et al. 2005 [72] |
Protein names and putative functions were partly assigned on sequence similarity and/or phylogenetic trees; please refer to the cited literature and references therein. Cys position of identified cysteine, Org organism, Cat catalytic cysteine, Ref literature reference, At Arabidopsis thaliana, Cr Chlamydomonas reinhardtii, Os Oryza sativa, Ps Pisum sativum, Pt Populus trichocarpa