Fig. 3.

HDAC6 inhibition suppresses migration of fibroblast-like synoviocytes (FLS). a Cell migration assay. RA-FLS (n = 6) were treated for 1 h with M808, followed by stimulation with IL-1β for 72 h. Migration of RA-FLS in the presence of varying concentrations of M808 is depicted. Shown are representative images from six independent experiments (left panel). Original magnification × 40. The number of migrating RA-FLS in each group was compared using a t-test (right panel). b Following treatment with IL-1β in the presence or absence of M808 (5 μM), FLS were stained for F-actin (green) and cortactin (red). Nuclei were stained with DAPI (blue). Immunofluorescence images are representative of five independent experiments; invadopodia (yellow dots) are seen in the merged panels. Bar = 20 μm. The number of invadopodia per cell was compared between groups using a t-test (right panel). Data represented the mean ± SEM