Fig. 4.

M808 inhibits chemotaxis and adhesion of inflammatory cells to RA-fibroblast-like synoviocytes (FLS). a RA-FLS (n = 6) were pre-treated with M808 for 1 h, followed by stimulation with IL-1β for 24 h. The amounts of CCL-2, CXCL-8, and CXCL-10 in culture supernatants were measured in an ELISA. b ICAM-1 and VCAM-1 expression by RA-FLS was examined by Western blotting. The image is representative of three independent experiments (left panel). The expression of ICAM-1 and VCAM-1 relative to GAPDH were quantified (middle and right panels). Data represent the mean ± SEM. c, d Adhesion of calcein-labeled U937 and Jurkat cells to RA-FLS after treatment with the HDAC6 inhibitor (n = 6). Data represent the mean ± SEM. *p < 0.05 vs. cells treated with IL-1β. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM, intercellular adhesion molecule; VCAM, vascular cell adhesion molecule; Tuba, tubastatin A