Table 4.
ORF3a mutants compared to the wild type. The percentage of the peak area of the APRs is shown. The ΔG difference is associated with the destabilization of the protein's structure. Underlined ΔG show the analyzed variants.
| ORF3a Variant | % Peak Area | Energy Difference (kcal/mol) |
|---|---|---|
| Wild type | 65.91 | 0.0 |
| QNG41554.1 USA (L53F) | 72.24 | 2.2044 |
| QNG41770.1 USA (L140F) | 66.80 | 2.1808 |
| QND77223.1 USA (Q57H, P104S) | 67.97 | 2.4939 |
| QNA38024.1 USA (Q57H, Y160H) | 69.82 | 5.0052 |
| QNA38792.1 USA (Q57H, I123V) | 70.09 | 1.5448 |
| QMU94777.1 Bangladesh (S171L) | 69.53 | 11.2476 |
| QMI98320.1 USA (Q57H, Q185H) | 69.55 | 1.3686 |
| QLM05656.1 USA (G172C) | 68.89 | 4.3654 |
| QLM05764.1 USA (L53H, Q57H) | 67.44 | 2.9557 |
| QLH56255.1 Saudi Arabia (Q57H, S216P) | 66.35 | 3.3689 |
| QKS66941.1 Egypt (V55F, S171L) | 69.13 | 9.9923 |
| QKN20824.1 USA (Q57H, A59V) | 65.81 | 1.0337 |
| QKG90399.1 USA (Q57H, R134C) | 71.74 | 1.5122 |
| QKG64052.1 USA (F56C) | 71.57 | 1.2334 |
| QKE44990.1 USA (L94P, V97A, F120L) | 67.98 | 5.4940 |
| QJD47849.1 Taiwan (Q57H, M125I) | 74.34 | 1.2187 |
| QJD23418.1 USA (Q57H, A99D) | 67.94 | 1.0058 |